Harkiolaki Group at Diamond

B24 is a correlative cryo-imaging beamline based at Diamond Light Source, offering 3D imaging of the cellular landscape with soft X-ray tomography (cryoSXT) complemented by super resolution fluorescence structured illumination microscopy (cryoSIM). 

The technology available at beamline B24, Diamond Light Source, has enabled scientific discoveries in a number of biomedical areas of interest such as: (a) plasmodium egress mechanisms in infected human red blood cells, (b) reovirus vesicle escape in the early stages of infection, (c) the discovery of a new cytotoxic membrane-less organelle that killer T-cells deposit on the surface of their targets, (d) photo-activatable anti-cancer compound uptake, distribution and effect in human cancer cells, (e) SARS-CoV2 intracellular progress and egress in the late stages of infection, (f) distribution trends of Chlamydiae populations in intracellular inclusions in infected human cells and, (g) processing and expression of secondary antigen production in cells exposed to pathogen-derived RNA-carrier vaccines (see Appendix for relevant references).

Cell culture and associated molecular biology techniques are aided by facilities at the Research Complex at Harwell and at the beamline.