Shimadzu UV1800

The UV1800 is a simple UV spectrometer capable of samples between 200-700 nm. The samples are analysed using cuvettes, so this machine is only suitable for liquid samples or samples capable of being put in to suspension.

Both the UV1800 and UV2600 use Shimadzu UV Probe software for method development, data capture and analysis. The data can be exported as a .csv file to allow for more data processing offline.

UV Spectroscopy, also known as UV-Visible Spectrometry (UV/Vis or UV-Vis), is a technique that looks at the amount of energy absorbed by the sample in the UV to visible light spectrum compared to a reference sample. The light source subjects the sample to discrete wavelengths over the typical range 200-1000 nm, and looks at the amount of energy absorbed due to the excitation of electrons within the sample when the frequency of the incident light corresponds to the energy difference of the electronic transition. This absorption gives insight into the structure/bonding of the subject material. This resultant energy difference between the sample and reference is examined across the UV-Vis light range and the spectra produced. This is most commonly looked at in absorbance (A), but can also be looked at in transmission (%T) and reflectance (%R).