The UV1800 is a simple UV spectrometer capable of samples between 200-700 nm. The samples are analysed using polystyrene or quartz cuvettes, this machine is only suitable for liquid samples or samples suspended in liquid.
The UV1800 use Shimadzu UV Probe software for method development, data capture and analysis. The data can be exported as a .csv file to allow for offline data processing.
UV Spectroscopy, (UV/Vis or UV-Vis), is a technique that looks at the amount of energy absorbed by the sample in the UV to visible light spectrum compared to a reference material. The light source subjects the sample to discrete wavelengths over the typical range 200-1000 nm, and looks at the amount of energy absorbed due to the excitation of electrons within the sample when the frequency of the incident light corresponds to the energy difference of the electronic transition. This absorption gives insight into the structure/bonding of the subject material. This resultant energy difference between the sample and reference is examined across the UV-Vis light range and the spectrum produced. This is most commonly recorded in absorbance (A), but can also be measured in transmission (%T) and reflectance (%R).
