Speaker – Dr. Eduardo Pasca
25th January 2022, 11:00 BST
The Collaborative Computational Project in Tomographic Imaging (CCPi) Core Imaging Library (CIL) is a versatile Python package for tomographic imaging intended for CT experimentalists to easily access optimised standard algorithms. Bespoke pipelines can be created to handle novel imaging rigs, dynamic, hyperspectral, nonstandard scan geometry, to name just a few. CIL is also intended for imaging specialists to allow the creation of novel reconstruction algorithms and assess them against established ones. CIL seamlessly handles the CT pipeline from loading data from common lab X-Ray CT machines, as NIKON or ZEISS, to FDK or iterative reconstruction. CIL also provides utilities for visualisation and exploration of the data.
In this seminar, Dr Edoardo Pasca will talk about some examples of applications of the methods available with CIL.
Don’t forget to register via Eventbrite and pass this information on to your contacts, thanks.
Past Event Recordings:
Seminar Series at Research Complex at Harwell
Title – Measuring what really matters: affinity determination in complex samples and with challenging proteins
Speaker – Dr. Sean Devenish
4th November 2021, 10:30-11.30 GMT
Fluidic Analytics is a commercial biotech company originating from the University of Cambridge, established in 2013, and headquartered in Cambridge, UK. Fluidic Analytics provides a unique technology, Microfluidic Diffusional Sizing (MDS), that allows characterisation of proteins and their interactions, including measurements of size, concentration, stoichiometry and binding affinity. Importantly, measurements are performed fully in solution and can be taken directly in complex backgrounds such as cell lysates, serum or plasma. These features have led to MDS yielding valuable data in the area of infectious diseases and immune profiling (antibody detection, affinity/antibody maturation, virus neutralisation, or cross-reactivity studies) as well as studies on challenging proteins (membrane proteins, fibrils, IDPs, etc.).
- Introduction to Microfluidic Diffusional Sizing
- Case study 1: COVID-19: quantitative information the concentration and affinity of antibodies in polyclonal serum samples provides deeper understanding of the immune response to SARS-CoV-2
- Case study 2: Amyloid beta peptide: measuring binding to monomeric and fibrillar forms provides useful insights into the mechanism of action of therapeutic antibodies
- Case study 3: beyond protein-protein interactions: MDS with SMALPs, aptamers, and small molecules
Dr Sean Devenish is Head of Technical Support and Field Applications at Fluidic Analytics. He is responsible for scientific and technical support, as well as delivery of the laboratory services business. He has a PhD in chemistry from the University of Canterbury, New Zealand, and over a decade of postdoctoral experience in biochemistry, biophysics and protein engineering, including six years at the University of Cambridge. Sean has published more than 30 peer-reviewed scientific publications including two in Nature journals. He joined Fluidic Analytics six years ago as the second employee and led the R&D team before moving to the commercial function where he uses his broad and deep knowledge of proteins, assays and our technology to support our valued customers.