Surface plasmon resonance (SPR) is used to study molecular interactions. One interacting partner is attached to the surface of a sensor chip and then the other interaction partner is passed over the surface. Binding of molecules to the sensor surface generates a response that is proportional to the bound mass. The association and dissociation of the bound molecules is followed in real time. The technique is applicable to almost any type of molecular interaction from sub-100Da molecules up to whole cells, viruses and nanoparticles, providing high-quality data on binding affinity, kinetics and specificity, as well as concentration measurements based on specific binding activity. Research Complex has a Biacore T200 SPR system.
Surface Plasmon Resonance
