Stopped-flow is used to study reactions on the milliseconds timescale. Research Complex has a TgK Scientific SF61DX2 stopped-flow system. The system can detect both absorbance and fluorescence signals, and has an optional scanning CCD detector for use in multi-wavelength experiments. It can also measure fluorescence anisotropy, which can be used to measure the binding constant for an interaction. The instrument can operate in single- or double-mixing mode, the latter enabling use of an optional quench-flow module that allows the user to isolate intermediates and products within a reaction mechanism and take them away for analysis by other means. This is a complementary technique to stopped-flow and can be used when the reaction does not produce spectroscopic changes, or when spectroscopic changes cannot identify specific reaction components.
Stopped-flow fluorimeter
