Super-resolution fluorescence microscopy now available at the RCaH

The Lasers for Science Facility has built a bespoke fluorophore localisation microscope for carrying out the family of super-resolution techniques that includes stochastic optical reconstruction microscopy (STORM) and photoactivated localisation microscopy (PALM). This enables imaging beyond the diffraction limit, with 2D resolution down to 50 nm in the membranes of cells. The microscope is equipped with four excitation wavelengths (405, 488, 561 and 640 nm) and uses total internal reflection to maximise the resolution in superficial sample planes. This microscope will be available for access from the next LSF call (December 2012). For more information click here