Light Scatterer: Wyatt Dawn Helios II

The interaction of light with matter can give information on the structure and dynamics of that material. Light scattering is a non-invasive technique for characterizing macromolecules and a wide range of particles in solution. In contrast to most methods for characterization, it does not require outside calibration standards.

The Wyatt Dawn Helios II Light Scatterer makes 2 types of measurement for absolute molecular characterization:

  • Classical Light Scattering / Static Light Scattering: Here, the intensity of the scattered light is measured as a function of angle. For the case of macromolecules, this is often called Rayleigh scattering and can yield the molar mass, rms radius, and second virial coefficient (A2). For certain classes of particles, classical light scattering can yield the size, shape, and structure.
  • Quasi-elastic Light Scattering (QELS) or Dynamic Light Scattering (DLS): In a QELS measurement, time-dependent fluctuations in the scattered light signal are measured using a fast photon counter. QELS measurements can determine the hydrodynamic radius of macromolecules or particles.

For more information from the manufacturer, click here or here.